GSK3B (Glycogen synthase kinase 3 beta, Serine/Threonine-Protein Kinase GSK3B, Glycogen Synthase Kinase-3 Beta)

Size

£685.00

SKU:: 046127
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to human GSK-3alpha.
Clone:: 9B1-5C3-5G8
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide
Extra Details:: GSK3B is a serine-threonine kinase and a member of the glycogen synthase kinase subfamily. It is a negative regulator of the hormonal control of glucose, Wnt signaling and also transcriptional factors and microtubules. It has been associated with regulation of several cellular mechanisms such as glycogen synthesis, protein synthesis, cell proliferation, microtubules stabilization, cell migration, autophagy and apoptosis. GSK3B is activated by phosphorylation at tyrosine 216 and inactivated by phosphorylation at serine 9. Defects on GSK3B has been associated with Alzheimer’s and Parkinson’s diseases. Applications: Suitable for use in Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunocytochemistry: 1.25-5ug/ml. 4% PFA fixation followed by permeabilization with ice-cold methanol, or fixation/permeabilization with ice cold methanol alone is recommended. We do not recommend 4% PFA fixation followed by permeabilization with 0.5% Triton-X. Immunohistochemistry (FFPE): 4-6ug/ml, antigen retrieval using citrate buffer, HIER, and overnight antibody incubation at 4°C. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice