Nuclear Pore Complex Proteins

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£576.00

SKU:: 046122
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Nuclear pore complex mixture.
Clone:: MAb414
Format:: Supplied as a liquid in PBS, 0.03% thimerosal.
Extra Details:: Nuclear pores are large protein complexes that cross the nuclear envelope. The proteins that make up the nuclear pore complex are known as nucleoporins. About half of the nucleoporins typically contain solenoid protein domains—either an alpha solenoid or a beta-propeller fold, or in some cases both as separate structural domains. Each NPC contains at least 456 individual protein molecules and is composed of 30 distinct proteins (nucleoporins). The other half show structural characteristics typical of "natively unfolded" or intrinsically disordered proteins, i.e. they are highly flexible proteins that lack ordered secondary structure. These disordered proteins are the FG nucleoporins, so called because their amino-acid sequence contains many phenylalanine—glycine repeats. 046122 is a reliable general purpose monoclonal antibody which recognizes a related family of NPC proteins. This antibody is ideal for studying the morphology and composition of the nucleus and nuclear envelope. It is also useful in studying changes in the nuclear structure during mitosis and meiosis. Applications: Suitable for use in Western Blot, Immunocytochemistry, Immunoprecipitation, Immunohistochemistry and Immunoelectron Microscopy. Other applications not tested. Recommended Dilutions: Immunocytochemistry (IF): 0.5-1ug/ml (1:1000-1:2000) Immunohistochemistry (formalin-fixed paraffin-embedded tissue): 0.5-1ug/ml Immunohistochemistry (frozen): 1:5000 Western Blot: 0.2-0.5ug/ml (1:2000-1:5000) Immunoprecipitation: 1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice