CD43 (CD43 Antigen, Galactoglycoprotein, GALGP, GPL115, Leukocyte Sialoglycoprotein, Leukosialin, LSN, Sialophorin, SPN) (Biotin)

Size

£550.00

SKU:: 046107
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Mouse WEHI 274.3 myeloid tumor cells
Clone:: 1B11
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Labeled with Biotin.
Extra Details:: CD43, also known as Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, and gp115, is a large single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites. Due to variable glycosylation and sialylation, two isoforms of CD43 have been identified. The 115kD glycoform of CD43 is expressed on most hematopietic cells including T lymphocytes, NK cells, monocytes, granulocytes, platelets, and CD5+ B cells. It does not present on resting B cells and erythrocytes. While the 130kD glycoform is thought to be activation-associated form primarily expressed on myeloid cells, pre-B cells, and activated T cells. It has been reported that CD43 binds to CD54 and Siglec-1. CD43 plays dual roles in cell adhesion and anti-adhesion, as well as costimulation of T cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors. This antibody reacts with the activation-associated glycoform of CD43. The epitope recognized by this monoclonal antibody is also shared with desialylated CD45RB. This antibody is useful for differentiation of effector CD8 T cells and memory T cells. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Flow Cytometry: ≤0.25ug/10e6 cells in 100ul volume Immunohistochemistry: acetone-fixed frozen sections. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice