046106
FceRI, alpha (Fc epsilon RI alpha, FCE1A, FCER1A, High Affinity Immunoglobulin epsilon Receptor Subunit alpha, IgE Receptor) (PE)
Size
£692.00
- SKU:
- 046106
- Application:
- Flow Cytometry
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Clone:
- AER-37 (CRA-1)
- Format:
- Supplied as a liquid in PBS, pH 7.2, BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
- Extra Details:
- High affinity IgE receptor (FceRI) plays a key role in IgE-mediated allergic immune response. FceRI is a tetrameric receptor complex, which is composed of one a-subunit (FceRIa), one B-subunit, and two y-subunits. FceRIa directly binds IgE with high affinity, while the B- and y-chains are responsible for mediating intracellular signals. FceRIa is a 50kD transmembrane protein with Ig superfamily structure. It is primarily found on mast cells and basophils. Further studies have indicated that FceRIa is also expressed on many inflammatory cells including cutaneuos Langerhans cells, dendritic cells, monocytes of patients with allergic disorders, platelets, bronchial epithelial cells, eosinophils produced in hypereosinophilic syndrome, and neutrophils from allergy-induced asthma patients. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 5ulg/10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice