046072
Fibrinogen (Coagulation Factor I) (HRP)
Size
£539.00
- SKU:
- 046072
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Sheep
- Immunogen:
- Fibrinogen purified from human plasma
- Format:
- Supplied as a liquid in a buffered stabilizer solution, 50% glycerol. Labeled with horseradish peroxidase (HRP).
- Extra Details:
- Fibrinogen is an abundant plasma protein (5-10uM) produced in the liver. The intact protein has a molecular weight of 340kD and is composed of 3 pairs of disulfide-bound polypeptide chains named A-alpha, B-beta and gamma. Fibrinogen is a triglobular protein consisting of a central E domain and terminal D domains. Proteolysis by thrombin results in release of Fibrinopeptide A (FPA, A-alpha1-16) followed by Fibrinopeptide B (FPB, B-beta1-14) The resulting fibrin monomers polymerize in a half-overlap to form insoluble fibrin fibrils. The terms alpha, beta and gamma are used to indicate the removal of FPA and FPB. Polymerized fibrin is stabilized by the transglutaminase activated Factor XIII that forms amide linkages between gamma chains and, to a lesser extent, alpha chains of the fibrin molecules. Proteolysis of fibrinogen releases C-terminal residues from the A-alpha chain to produce fragment X (intact DE-D, which still clots). Fragment X is further degraded to non-clotting fragments Y (D-E) and D. Fragment Y can be digested into its components: D and E fragments. Digestion of non-crosslinked fibrin with plasmin is very similar to the digestion of fibrinogen, which results in production of fragments D and E. Degradation of crosslinked fibrin by plasmin results in fragment DD (D-Dimer consisting of the D domains of 2 fibrin molecules crosslinked via the gamma chains), fragment E (central E domain) as well as DDE in which fragment E is non-covalently associated with DD. For human crosslinked fibrin, the relative weights of the cleavage fragments produced are: 184kD for fragment DD, 92kD for D, 50kD for E, 1.54kD for FPA and 1.57kD for FPB. Application: Suitable for use ELISA and Immunoelectrophoresis. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice