IL-17A, Recombinant, Mouse, His-Tag, aa26-158

Size

£462.00

SKU:: 046061
Molecular Weight:: 16
Purity:: ≥95% (SDS-PAGE). Endotoxin: ≤1EU/1ug (LAL).
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.
Extra Details:: IL-17A, also known as CTLA‑8, is a cytokine that belongs to the Interleukin‑17 family. It produced by activated T cells and plays an important role in anti‑microbial and chronic inflammation. Mouse IL‑17A shares 61% amino acid sequence identity with human IL‑17A. It promotes protective mucosal and epidermal inflammation in response to microbial infection and induces chemokine production, neutrophil influx, and the production of antibacterial peptides. It can stimulate the expression of IL-6 and PTGS2/COX-2, as well as enhance the production of nitric oxide(NO). High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis, and multiple sclerosis. Also, IL‑17A has been shown to exert either tumorigenic or anti‑tumor effects. Recombinant mouse IL-17A, fused to His-Tag at C-terminal, was expressed in insect cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa26-158 from mouse IL-17A, fused to His-Tag at C-terminal, expressed in Baculovirus. Molecular Weight: ~16kD (141aa) Amino Acid Sequence: AAIIPQSSAC PNTEAKDFLQ NVKVNLKVFN SLGAKVSSRR PSDYLNRSTS PWTLHRNEDP DRYPSVIWEA QCRHQRCVNA EGKLDHHMNS VLIQQEILVL KREPESCPFT FRVEKMLVGV GCTCVASIVR QAA<LEHHHHH H> Biological Activity: The activity is determined by the IL-6 ELISA in a using NIH/3T3 mouse embryonic fibroblast cells. The ED50 range ≤ 2ng/ml. Applications: Suitable for use in SDS-PAGE, Bioactivity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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