CD14, Recombinant, Human, His-Tag, aa20-344

Size

£439.00

SKU:: 046040
Molecular Weight:: 35.8
Purity:: ≥95% (SDS-PAGE). Endotoxin: ≤1EU/1ug (LAL).
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.
Extra Details:: CD14, also known as monocyte differentiation antigen CD14, is a member of the eucine-rich repeat (LRR) proteins family. It exists in two forms, one anchored to the membrane by a GPI-tail (mCD14), the other a soluble form (sCD14). It is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide(LPS). It acts as a co_x0002_receptor (along with TLR4 and MD-2) and helps to detect bacteria in the body by binding LPS, a pathogen_x0002_associated molecular pattern (PAMP). Recombinant human CD14, fused to His-Tag at C-terminal, was expressed in HEK293 cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa20-344 from human CD14, fused to His-Tag at C-terminal, expressed in HEK293. Molecular Weight: ~35.8kD (331aa) Amino Acid Sequence: TTPEPCELDD EDFRCVCNFS EPQPDWSEAF QCVSAVEVEI HAGGLNLEPF LKRVDADADP RQYADTVKAL RVRRLTVGAA QVPAQLLVGA LRVLAYSRLK ELTLEDLKIT GTMPPLPLEA TGLALSSLRL RNVSWATGRS WLAELQQWLK PGLKVLSIAQ AHSPAFSCEQ VRAFPALTSL DLSDNPGLGE RGLMAALCPH KFPAIQNLAL RNTGMETPTG VCAALAAAGV QPHSLDLSHN SLRATVNPSA PRCMWSSALN SLNLSFAGLE QVPKGLPAKL RVLDLSCNRL NRAPQPDELP EVDNLTLDGN PFLVPGTALP HEGSM<HHHHH H> Biological Activity: Measured by its ability to enhance IL-8 secretion using THP‑1 human acute monocytic leukemia cells in the presence of LPS. The ED50 range ≤ 100ng/ml. Applications: Suitable for use in SDS-PAGE, Bioactivity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice