IgG1 Fc, Recombinant, Human, aa100-330

Size

£386.00

SKU:: 046039
Molecular Weight:: 25.9
Purity:: ≥90% (SDS-PAGE). Endotoxin: ≤1EU/1ug (LAL).
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.
Extra Details:: Immunoglobulins are membrane-bound or secreted glycoproteins produced by B lymphocytes. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as the antibody-dependent cell-mediated cytotoxicity. IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors on phagocytic cells with high affinity. IgG comprise two identical light (L) and heavy (H) chains forming a ‘Y‐shaped’ structure. It also is digested by pepsin proteolysis into two distinct fragments (Fab, Fc). Fc fragment is a homodimeric glycoprotein with one monomer consisting of two constant immunoglobulin domains (CH2, CH3) from the C‐terminal half of one heavy chain. Through interaction with its ligands (FcγR, C1q and FcRn) it mediates various immune effector functions and increases the half‐life of the antibody molecule. Recombinant IgG Fc is suggested to represent a potential anti-inflammatory drug for treatment of human autoimmune diseases. Recombinant human IgG1 Fc was expressed in HEK293 cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa100-330 from human IgG1 Fc, expressed in HEK293. Molecular Weight: ~25.9kD (231aa) Amino Acid Sequence: PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG K Biological Activity: 1. Measured by its binding ability in a functional ELISA with Human Fc gamma RIIIA/CD16a. The ED50 range ≤ 1.5ug/ml. 2. Measured by its binding ability in a functional ELISA with Human Fc gamma RIIIA/CD16a. 3. Measured by its binding ability in a functional ELISA with Human Fc gamma RIIIA/CD16a. The ED50 range ≤ 4ug/ml. Applications: Suitable for use in SDS-PAGE, Bioactivity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice