Epiregulin, Recombinant, Human, His-Tag, aa63-108

Size

£512.00

SKU:: 046012
Molecular Weight:: 32.6
Purity:: ≥90% (SDS-PAGE). Endotoxin: ≤1EU/1ug (LAL).
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.
Extra Details:: Epiregulin is a member of the epidermal growth factor family. It is expressed primarily in the placenta and macrophages and high level expression has also been detected in various carcinomas. This protein contributes to inflammation, wound healing, tissue repair, and oocyte maturation by regulating angiogenesis and vascular remodeling and by stimulating cell proliferation. Epiregulin is growth factors involved in cancer development. Deregulated epiregulin activity appears to contribute to the progression of a number of different malignancies, including cancers of the bladder, stomach, colon, breast, lung, head and neck, and liver. It inhibit the growth of several epithelial tumor cells and stimulated the growth of fibroblasts and various other types of cells. In addition, it has been implicated in the implantation process during pregnancy. Recombinant human Epiregulin, fused to hIgG-His-Tag at C-terminal, was expressed in HEK293 cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa63-108 from human Epiregulin, fused to hlgG-His-Tag at C-terminal, expressed in HEK293. Molecular Weight: ~32.6kD (289aa) Amino Acid Sequence: <DGSM>VSITKC SSDMNGYCLH GQCIYLVDMS QNYCRCEVGY TGVRCEHFFL <LEPKSCDKTH TCPPCPAPEL LGGPSVFLFP PKPKDTLMIS RTPEVTCVVV DVSHEDPEVK FNWYVDGVEV HNAKTKPREE QYNSTYRVVS VLTVLHQDWL NGKEYKCKVS NKALPAPIEK TISKAKGQPR EPQVYTLPPS RDELTKNQVS LTCLVKGFYP SDIAVEWESN GQPENNYKTT PPVLDSDGSF FLYSKLTVDK SRWQQGNVFS CSVMHEALHN HYTQKSLSLS PGKHHHHHH> Biological Activity: Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 range ≤1ug/ml. Applications: Suitable for use in SDS-PAGE, Bioactivity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice