IL-21R, Recombinant, Mouse, His-Tag, aa20-237

Size

£462.00

SKU:: 046003
Molecular Weight:: 52
Purity:: ≥95% (SDS-PAGE). Endotoxin: ≤1EU/1ug (LAL).
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.
Extra Details:: IL-21R (Interleukin 21 receptor), also known as NILR, belongs to the type I cytokine receptor family and Type 4 subfamily. This receptor is Heterodimer with the common gamma subunit and transduces the growth promoting signal of IL21. It is important for the proliferation and differentiation of T cells, B cells, and NK cells. The ligand binding of this receptor leads to the activation of multiple downstream signaling molecules, including JAK1, JAK3, STAT1, and STAT3. B cell IL‑21 R engagement induces Blimp-1, and is important for memory responses. It engagement on mouse NK cells enhances their cytotoxic activity and IFN-gamma production. It is selectively expressed in lymphoid tissues and most highly expressed in thymus and spleen and IL21 impacts some cell types, including CD8+ memory T-cells, NK cells, and subsets of CD4 memory T cells. It is also necessary for sufficient numbers of regulatory T cells to combat chronic inflammation. Recombinant mouse IL21R, fused to hIgG-His-Tag at C-terminal, was expressed in HEK293 cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa20-237 from mouse IL-21R, fused to hlgG-His-Tag at C-terminal, expressed in HEK293. Molecular Weight: ~52kD (457aa) Amino Acid Sequence: CLDLTCYTDY LWTITCVLET RSPNPSILSL TWQDEYEELQ DQETFCSLHR SGHNTTHIWY TCHMRLSQFL SDEVFIVNVT DQSGNNSQEC GSFVLAESIK PAPPLNVTVA FSGRYDISWD SAYDEPSNYV LRGKLQYELQ YRNLRDPYAV RPVTKLISVD SRNVSLLPEE FHKDSSYQLQ VRAAPQPGTS FRGTWSEWSD PVIFQTQAGE PEAGWDPH<LE PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG KHHHHHH> Biological Activity: Measured by its ability to inhibit IFN-g secretion using NK-92 human natural killer cells. The ED50 range ≤ 4 ug/ml with mouse IL-2. Applications: Suitable for use in SDS-PAGE, Bioactivity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice