rnpA, Recombinant, E. coli, aa1-119

Size

£479.00

SKU:: 046001
Molecular Weight:: 13.7
Purity:: ≥90% (SDS-PAGE). Endotoxin: ≤1EU/1ug (LAL).
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.
Extra Details:: rnpA, also known as Rnase P protein, is an essential enzyme consisting of the C5 protein (encoded by rnpA) and the catalytic M1 RNA (encoded by rnpB) subunits. rnpA is ribonucleoprotein that catalyzes the removal of the 50-leader elements of precursor tRNAs and generates the mature 50-end of tRNAs. This step is essential for the formation of functional tRNA molecules in bacteria, archaea and eukarya. More importantly, it has recently been demonstrated that RNase P is required for the endonucleolytic separation of certain polycistronic tRNA transcripts such as valV valW, leuQ leuP leuV and secG leuU. Thus, it was hypothesized that the essential function of RNase P might be related to the complete absence of a particular tRNAthat was dependent on the enzyme for initial separation from polycistronic transcripts. Recombinant E.coli rnpA, was expressed in E. coli and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa1-119 from e.coli rnpA, expressed in E.coli. Molecular Weight: ~13.7kD Amino Acid Sequence: MVKLAFPREL RLLTPSQFTF VFQQPQRAGT PQITILGRLN SLGHPRIGLT VAKKNVRRAH ERNRIKRLTR ESFRLRQHEL PAMDFVVVAK KGVADLDNRA LSEALEKLWR RHCRLARGS Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice