Selenoprotein W, Recombinant, Human, His-Tag, aa1-87

Size

£439.00

SKU:: 045980
Molecular Weight:: 11.8
Purity:: ≥95% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.15 Sodium Chloride, 1mM DTT, 20% glycerol.
Extra Details:: SEPW1 is a selenoprotein, which contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the uGA codon that normally signals translation termination. The 3 uTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of uGA as a Sec codon rather than as a stop signal. This protein shows highest expression in skeletal muscle and heart, and may be involved in oxidation-reduction reactions. A retroprocessed pseudogene, SEPW1P, has been identified and mapped to chromosome 1p35-34. Recombinant human SEPW1 protein, fused to His-Tag at N_x0002_-terminal, was expressed in E. coli and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa1-87 from human Selenoprotein W, fused to His-Tag at N-terminal expressed in E.coli. Molecular Weight: ~11.8kD (110aa) Amino Acid Sequence: <MGSSHHHHHH SSGLVPRGSH MGS>MALAVRV VYCGACGYKS KYLQLKKKLE DEFPGRLDIC GEGTPQATGF FEVMVAGKLI HSKKKGDGYV DTESKFLKLV AAIKAALAQG Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice