Adiponectin (Acrp30, GBP-28, apM1, AdipoQ) (Biotin)

Size

£1,010.00

SKU:: 045949
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human Adiponectin.
Clone:: 112-1
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Labeled with Biotin.
Extra Details:: Adiponectin, also referred as Acrp30, AdipoQ, apM1, and gelatin binding protein of 28 kD (GBP-28), is encoded by the ADIPOQ gene in humans. Adiponectin contains 244 amino acids with a collagen-like domain at the N-terminal and a globular domain at the C-terminal. The globular domain can be proteolytically truncated forming the globular adiponectin (gAdiponectin). This globular domain resembles the structure of the complement C1q and the TNF-α superfamily. Adiponectin exists in a wide range of homo-oligomers: a low-molecular weight (LMW) trimer, a medium molecular weight (MMW) hexamer, and a high molecular weight (HMW) multimer with little or no interconversion between the forms. Females generally have higher HMW than males in proportion and absolute amounts. Adiponectin has two receptors (adipoRs)-1 and -2. AdipoR1 mRNA is detectable in skeletal muscle, spleen, lung, heart, kidney, and liver. AdipoR2 is expressed in liver, but is also detectable in heart, lung, skeletal muscle, and kidney. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA (detection): 0.05-0.2ug/ml. To obtain a linear standard curve, serial dilutions of Adiponectin protein ranging from 10,000-156 pg/ml are recommended for each ELISA plate. Optimal dilutions to be determined by the researcher. Matched Pair: Detection: 045949 Capture: 045948 Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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