Adiponectin (Acrp30, GBP-28, apM1, AdipoQ)

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£744.00

SKU:: 045948
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human Adiponectin.
Clone:: 62-9
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Extra Details:: Adiponectin, also referred as Acrp30, AdipoQ, apM1, and gelatin binding protein of 28 kD (GBP-28), is encoded by the ADIPOQ gene in humans. Adiponectin contains 244 amino acids with a collagen-like domain at the N-terminal and a globular domain at the C-terminal. The globular domain can be proteolytically truncated forming the globular adiponectin (gAdiponectin). This globular domain resembles the structure of the complement C1q and the TNF-α superfamily. Adiponectin exists in a wide range of homo-oligomers: a low-molecular weight (LMW) trimer, a medium molecular weight (MMW) hexamer, and a high molecular weight (HMW) multimer with little or no interconversion between the forms. Females generally have higher HMW than males in proportion and absolute amounts. Adiponectin has two receptors (adipoRs)-1 and -2. AdipoR1 mRNA is detectable in skeletal muscle, spleen, lung, heart, kidney, and liver. AdipoR2 is expressed in liver, but is also detectable in heart, lung, skeletal muscle, and kidney. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA (capture): 0.5-2ug/ml. To obtain a linear standard curve, serial dilutions of Adiponectin protein ranging from 10,000-156 pg/ml are recommended for each ELISA plate. Optimal dilutions to be determined by the researcher. Matched Pair: Detection: 045949 Capture: 045948 Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice