TNAP (Tissue Non-specific Alkaline Phosphatase, MSCA-1, Liver/Bone/Kidney Alkaline Phosphatase)

Size

£512.00

SKU:: 045944
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: WERI-RB-1 retinoblastoma cell line
Clone:: W8B2B10
Format:: Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Extra Details:: Tissue non-specific alkaline phosphatase (TNAP), also known as liver/bone/kidney alkaline phosphatase, or MSCA-1 (mesenchymal stem cell antigen 1) is a selective marker for the prospective isolation of bone marrow-derived mesenchymal stem cells and mesenchymal stem-like cells. It is expressed at high levels in liver, bone, kidney, or endometrium, as well as on embryonic stem cells (ESCs). TNAP also plays a role in bone mineralization. Mutations in TNAP gene are associated with hypercalcemia and skeletal defects (hypophosphatasia). Applications: Suitable for use in Flow Cytometry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1-4ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.