045890
Apolipoprotein E,E2,E3,E4 (Apo E,E2,E3,E4) (PE)
Size
£748.00
- SKU:
- 045890
- Application:
- Flow Cytometry
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to amino acids surrounding the polymorphic amino acid position 158 of Apo-E.
- Clone:
- 2B16 (E6D7)
- Format:
- Supplied as a liquid in PBS, pH 7.2, BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
- Extra Details:
- Apo-E is a class of apolipoprotein primarily synthesized in the liver, but is also found in other tissues such as brain, kidneys, and spleen. Apo-E is a protein component of serum chylomicron and lipoprotein particles, playing essential roles in cholesterol transportation, homeostasis, and metabolism. The expression of Apo-E is induced by cholesterol-rich diets and hyperlipoproteinemia. In the central nervous system, Apo-E is produced by astrocytes and microglia. The Apo-E-containing lipoprotein particles play important functions in brain injury repair, neurogenesis, and synaptic plasticity. Apo-E has been found to be involved in the regulation of amyloid-β aggregation and clearance. Genetic polymorphisms of Apo-E are associated with neuron degenerative disorders including Alzheimer's disease. Applications: Suitable for use in Western Blot, ELISA, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Flow Cytometry (intracellular immunofluorescent staining): 5ul/10e6 cells in 100ul Optimal dilution determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice