NEURON SPECIFIC ENOLASE (NSE)

Size

£544.00

SKU:: 045875
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Chicken/Avian
Immunogen:: Full length recombinant protein corresponding to human NSE protein, expressed in E. coli.
Format:: Supplied as a liquid in PBS, 0.03% sodium azide, 50% glycerol.
Extra Details:: Neuron specific enolase (NSE) is the neuronal form of the enolase glycolytic enzymes that catalyze the interconversion of 2-phosphoglycerate and phosphoenolpyruvate. NSE has a molecular weight of 45kD, and is expressed in normal pituitary, pinealocytes, neuroendocrine cells of the lung, thyroid, parafollicular cells, adrenal medulla, Langerhans islet cells, Merkel cells of the skin, melanocytes, normal striated muscle, and hepatocytes. NSE is a useful marker for neuroblastoma, small cell lung carcinoma, and neuroendocrine cancers. Applications: Suitable for use in Western Blot, Immunofluorescence, Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunofluorescence (IC): 1:500-1:1000 Immunohistochemistry: 1:10,000 Western Blot: 1:5000-1:10,000. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.