SREBP-1 (Sterol Regulatory Element Binding Protein-1, SREBP1, Sterol Regulatory Element Binding Transcription Factor 1, SREBF1)

Size

£672.00

SKU:: 045861
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to residues surrounding Gly45 of human SREBP-1
Clone:: E9F4O
Format:: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Extra Details:: Sterol regulatory element–binding proteins (SREBPs) are basic helix-loop-helix–leucine zipper (bHLH-Zip) transcription factors. Inactive precursor forms of SREBPs are bound to endoplasmic reticulum (ER) membranes. When cells are starved for sterols, SREBPs move from the ER to the Golgi apparatus with the help of SREBP cleavage activating protein (SCAP). In the Golgi apparatus, precursor SREBPs are then sequentially cleaved by two proteases, site-1 protease (S1P) and site-2 protease (S2P). The released N-terminal domain that contains the bHLH-Zip region enters the nucleus and binds to sterol response elements (SREs) in the promoters of a variety of genes responsible for the synthesis of cholesterol, fatty acids, and other lipids, activating their expressions (1,2). Studies show that SREBP-1-dependent fatty acid homeostasis has a critical role in promoting the pro-tumor phenotype of M2-like tumor-associated macrophages (TAMs), and inhibition of SREBP-1 enhances the efficacy of immune checkpoint blockade (3). In addition, suppression of cholesterol biosynthesis by statins stimulates SREBP-1 activation and, therefore, induces TGF-β signaling, promoting the epithelial-to-mesenchymal transition in pancreatic ductal adenocarcinoma (4). Applications: Suitable for use in Western Blot and ChIP. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 ChIP: 1:200, use 2.5ul of antibody and 10ug of chromatin (~4×10e6 cells) per IP Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice