045837
Green Fluorescent Protein (GFP)
Size
£607.00
- SKU:
- 045837
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Chicken/Avian
- Immunogen:
- Recombinant Aequoria victoria GFP expressed in E. coli
- Format:
- Supplied as a liquid in PBS, 5mM sodium azide, 50% glycerol.
- Extra Details:
- The green fluorescent protein (GFP) is a 27kD protein isolated originally from the jellyfish Aequoria victoria. It has an endogenous fluorochrome activity with excitation maximum at 395nm and emission maximum at 509nm, which is similar to that of fluorescein (1,2). The GFP gene was sequenced and the origin of the fluorochrome by autocatalytic activity of certain amino acids was discovered (3,4). Much interest in GFP was generated when it was shown that fluorescence develops rapidly when the protein is expressed and requires only molecular oxygen and no other cofactors. As a result GFP can be expressed in fluorescent form in essentially any prokaryotic or eukaryotic cell (5). GFP has been engineered to produce a vast number of variously colored mutants including blue, cyan and yellow protein derivatives, BFP, CFP and YFP (6-9). GFP and other fluorescent proteins derived from other Cnidarians (jellyfish, coral and medusa) are widely used as tracers in transfection and transgenic experiments to monitor gene expression and protein localization in vivo and in in vitro. The crystal structure of GFP was determined (7) which allowed amino acid modifications to improve spectral properties and prevent multimerization (8,9). GFP was the basis of the 2008 Nobel prize in chemistry, specifically “for the discovery and development of the green fluorescent protein, GFP”. This antibody can be used to verify the expression, size and stability of both AcGFP and eGFP fusion proteins in western blotting experiments and to amplify GFP signals in tissues of transgenic animals. Applications: Suitable for use in Immunohistochemistry, Western Blot, Immunocytochemistry and Immunofluorescence. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000-1:5000 Immunofluorescence/Immunocytochemistry: 1:1000-1:5000 Immunohistochemistry: 1:1000-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice