Laminin gamma 1

Size

£819.00

SKU:: 045833
Application:: IHC-Fr
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Basement membrane protein preparation from mouse EHS tumor.
Clone:: A5
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Extra Details:: Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Variations in the expression of laminins have been observed in embryogenesis, organogenesis, post traumatic healing and cancer. Laminins have been provoked by the discovery of their ability to promote neurite regeneration. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively). The gamma 1 chain, formerly thought to be a beta chain, contains structural domains similar to beta chains, however, lacks the short alpha region separating domains I and II. Embryos of transgenic mice in which both alleles of the gamma 1 chain gene were inactivated by homologous recombination, lacked basement membranes, indicating that laminin, gamma 1 chain is necessary for laminin heterotrimer assembly. Applications: Suitable for use in Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (formalin-fixed, paraffin-embedded): 1:50-1:500 detected strong membrane staining of basement membrane of formalin-fixed, paraffin-embedded human tumor cells. Pretreat tissues using a citrate buffer, pH 6.0 and high heat epitope retrieval techniques. Optimal dilutions to be determined by the researcher. Positive Control: Tubular carcinoma of the colon and fibroneuroma Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice