Myozap (Myocardium Zonula Adherens Protein, GRINL1A Upstream Protein, Gup, MYZAP, MYOZAP)

Size

£834.00

SKU:: 045832
Purity:: Supernatant
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide of corresponding to human myozap (myocardium zonula adherens protein)
Clone:: 517.67
Format:: Supplied as a liquid, 0.09% sodium azide
Extra Details:: VP1 and VP2 are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in the nucleus and the cytoplasm. Epitope mapping experiments (Wo- bus et al., see below) identified aa726 to aa733 (C-terminus; common to all 3 VP proteins) as the specific binding region. The antibody is also useful for characterization of different stages of infection. Applications: Suitable for use in Immunofluorescence/Immunocytochemistry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: frozen and paraffin. For paraffin sections microwave treatment with 100mM Tris, pH 9.5, 5% urea buffer; 10 min at 120°C recommended Western Blot: 1:10-1:50 Optimal dilutions to be determined by the researcher. Positive Control: Myocard. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.