Cas9 (D10A), Nuclear Localization Sequence (NLS), Recombinant, Streptococcus pyogenes, His-Tag (CRISPR Associated Protein 9)

Size

£810.00

SKU:: 045805
Molecular Weight:: 160
Purity:: ≥80%
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 300mM sodium chloride, 1mM DTT, 200mM imidazole, 10% glycerol
Extra Details:: SpCas9(D10A)-NLS is a catalytic mutant of Cas9 that contains a nuclear localization sequence for optimal activity. Full length recombinant S. pyogenes SpCas9(D10A)-NLS (conserved hypothetical protein), fused to a C-terminal His-Tag, expressed in E. coli. Molecular Weight: ~160kD Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.