SNAP 25 (Synaptosomal-Associated Protein 25) (Ascites Free)

Size

£819.00

SKU:: 045798
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human brain proteins immunoprecipitated with EP10. Epitope: N-terminal half
Clone:: SP14
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride. No preservative added.
Extra Details:: Synaptosomal-associated protein 25 (UniProt P60880; also known as SNAP-25, SUP, Super protein, Synaptosomal-associated 25kD protein) is encoded by the SNAP25 (also known as SNAP) gene (Gene ID 6616) in human. SNAP-25 belongs to the superfamily of soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein (SNAP) receptor (SNARE) proteins involved in mediating vesicle fusion events. Target or t-SNAREs located in the membranes of target compartments form stable subcomplexes and engage vesicle or v-SNAREs in the membranes of transport vesicles to facilitate the fusion. SNAP-25 is a t-SNARE protein of the trans-SNARE complex that executes fusion between the synaptic vesicle and plasma membrane. SANP-25 does not have a transmembrane domain and is anchored to the cytosolic face of membranes via palmitoyl side chains covalently linked to cysteine residues. Applications: Suitable for use in Immunohistochemistry, Western Blot and Electron Microscopy. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml detected SNAP-25 in 10ug of human brain tissue lysate. Immunohistochemistry: paraffin Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice