Mad2 (MAD2L1, Mitotic Spindle Assembly Checkpoint Protein MAD2A)

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£761.00

SKU:: 045793
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: His-tagged recombinant protein corresponding to human Mad2.
Clone:: AS55-A12
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Extra Details:: Mad2, also known as MAD2, MAD2L1, or Mitotic spindle assembly checkpoint protein MAD2A, is a mitotic spindle assembly checkpoint protein. The spindle checkpoint makes sure that all the chromosomes are aligned at the metaphase plate before anaphase begins which means that all chromosomes are attached to the spindle before anaphase can start. The spindle checkpoint becomes triggered when the chromosome(s) are unaligned. Mad2 then binds to the anaphase promoting complex (APC) and CDC20. This complex prevents the cell from transitioning into anaphase and ultimately from cell death or disease. Applications: Suitable for use in Western Blot and Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml detected Mad2 in 10ug of HEK293 cell lyate. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice