DNA/RNA Oxidative Damage (8-OH-dG, 8-OHG, 8-oxo-G, 7,8-dihydro-8-oxoguanosine)

Size

£819.00

SKU:: 045792
Purity:: Purified by ammonium sulfate precipitation.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: 8-Hydroxy-2-deoxyguanosine
Clone:: 7E6
Format:: Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.02% sodium azide, 50% glycerol.
Extra Details:: 8-Hydroxy-2'-deoxyguanosine (8-OH-dG) and 8-hydroxyguanosine (8-OHG) are oxidized nucleosides and markers of DNA and RNA oxidative damage, respectively. Levels of 8-OH-dG increase in DNA in the presence of eugenol and hydrogen peroxide or copper sulfate. Levels of 8-OHG increase in response to hydrogen peroxide in HeLa cells in a concentration-dependent manner. 8-OHG has been detected in neurons in postmortem brain from patients with Alzheimer's or Parkinson's disease. Applications: Suitable for use in ELISA and Immunoprecipitation. Other applications not tested. Recommended Dilutions: ELISA: 1:1000 Immunoprecipitation: 10ug/reaction Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.