Synaptobrevin (VAMP)

Size

£563.00

SKU:: 045770
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Crude synaptic preparation of post mortem human brain.
Clone:: SP10
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Extra Details:: Synaptobrevins, also known as VAMPs, along with syntaxins and the synaptosomal-associated protein SNAP25, form the protein complex that is involved with the docking and fusion of synaptic vesicles with the presynaptic membrane. Synaptobrevins have a molecular weight of 18 kD, and are also involved in the formation of the SNARE complexes. The synaptobrevins are degraded by the protein tetanospasmin, from the tetanus-causing bacterium Clostridium tetani, as well as botulinum toxin from Clostridium botulinum. Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-10ug/ml Immunohistochemistry (paraffin): 1-10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.