DAP12 (KARAP)

Size

£758.00

SKU:: 045745
Purity:: Purified by ammonium sulfate precipitation.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to human DAP12 at the cytoplasmic domain.
Format:: Supplied as a liquid in PBS, 0.08% sodium azide.
Extra Details:: DAP12 is a transmembrane adaptor protein that is non-covalently associated with several cell surface receptors on natural killer (NK), myeloid, and presumably neuronal cells (Bakker, 2000, Campbell, 1999). DAP12 contains an ITAM domain that preferentially recruits the protein tyrosine kinase Syk to initiate signal transduction (McVicar, 1998). Deficiency of DAP12 results in reduced antigen presentation function by myeloid cells and defects in function of certain NK cell receptors in mice (Bakker. 2000, Tomasello, 2000), as well as presenile dementia and bone cysts in humans (Paloneva, 2000). Applications: Suitable for use in Immunoprecipitation and Western Blot. Other applications have not been tested. Recommended Dilutions: Immunoprecipitation: 2-4ug/mg of protein lysate Optimal dilutions to be determined by the researcher. Positive Control: NKp44 positive cells. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.