Bromodeoxyuridine (BrdU)

Size

£486.00

SKU:: 045724
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: 5-bromodeoxyuridine conjugated with hemocyanine.
Clone:: MoBu-1
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Extra Details:: Bromodexyuridine (BrdU) is a thymidine analog, which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. Applications: Suitable for use in Immunohistochemistry, Immunocytochemistry and Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cyotmetry: 1-2ug/ml. Intracellular staining. Immunohistochemistry: paraffin Immunocytochemistry: 2ug/ml Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.