EpCAM (CD326, Epithelial Cell Adhesion and Activating Molecule)

Size

£607.00

SKU:: 045715
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: NCI-H69 small cell lung carcinoma cells.
Clone:: VU1D9
Format:: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Extra Details:: Epithelial cell adhesion and activating molecule (EpCAM/CD326) is a transmembrane glycoprotein that mediates Ca2+-independent, homophilic adhesions on the basolateral surface of most epithelial cells. EpCAM is not expressed in adult squamous epithelium, but it is highly expressed in adeno and squamous cell carcinomas (1). Research studies identified EpCAM as one of the first tumor-associated antigens, and it has long been a marker of epithelial and tumor tissue. Investigators have shown that EpCAM is highly expressed in cancer cells (reviewed in 2,3). Applications: Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry and Immunofluorescence/Immunocytochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry (Fixed/Permeabilized): 1:400-1:1600 Flow Cytometry (Live): 1:400-1:1600 Immunohistochemistry (paraffin): 1:500 Western Blot: 1:1000 Immunofluorescence (IC): 1:800 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice