TRAIL, soluble, Recombinant, Human (TRAIL, Apo-2L, TNFSF10, CD253)

Size

£599.00

SKU:: 045370
Molecular Weight:: 24
Purity:: ≥98% (SDS-PAGE). Endotoxin: ≤0.01EU/ug (LAL test; Lonza).
Storage Conditions:: -20°C/-80°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM HEPES, pH 7.4, 150mM sodium chloride, 0.005% Tween 20, 1% sucrose, 1mM DTT.
Extra Details:: TNF-related apoptosis-inducing ligand (TRAIL; Apo2L;CD253; TNFSF10) is a type II transmembrane protein of about 34kD. Like most members of the tumor necrosis factor (TNF) superfamily of cytokines TRAIL can be cleaved at the cell surface by metalloproteases to form a soluble molecule. Active TRAIL forms trimers and specifically binds to five distinct receptors: TRAIL-R1 (DR4; Apo2;CD261; TNFRSF10A), TRAIL-R2 (DR5; KILLER; TRICK2A;TRICK2B; CD262; TNFRSF10B), TRAIL-R3 (DcR1;LIT; TRID; CD263; TNFRSF10C), TRAIL-R4 (DcR2; TRUNDD; CD264; TNFRSF10D), and osteoprotegerin (OPG; OCIF; TNFRSF11B). Trimerized TRAIL triggers apoptosis upon ligation of cell surface TRAIL-R1 and/or TRAIL-R2 by inducing the formation of the so-called multiprotein death-inducing signaling complex (DISC). Sequence: The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide. Specificity: Binds to human TRAIL receptors. Binding to OPG has not been tested experimentally. Biological Activity: Potent biologically active trimer. Induces apoptosis in a concentration range of 10-100ng/ml. Note: Does not require a cross-linking enhancer for its potent biological activity. ED50: 20ng/ml (BJAB cells). Note: To retain full activity the concentration of the aliquots should be at least 0.1mg/ml. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Dry Ice