TNF-alpha, Soluble, Recombinant, Human (Tumor Necrosis Factor Ligand Superfamily Member 2, Cachectin)

Size

£416.00

SKU:: 045342
Molecular Weight:: 17
Purity:: ≥95% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder from PBS pH 7.2. rReconstitute with 100ul sterile ddH2O.
Extra Details:: Tumor necrosis factor (TNF, cachexin or cachectin and formerly known as tumor necrosis factor-alpha) is a cytokine involved in systemic inflammation and is a member of a group of cytokines that stimulate the acute phase reaction. Recombinant protein corresponding to aa85-223 with a FLAG®-tag at the N-Terminus of human, expressed in E. coli Biological Activity: Induces cell death at a concentration range of 0.1-1ng/ml (ED50=0.05ng/ml or 2x10e7 units/mg; WEHI 164 cells). Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.