Sirtuin 5 (SIRT5, NAD-dependent Deacetylase Sirtuin-5, SIR2-like Protein 5, SIR2L5)

Size

£416.00

SKU:: 045297
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to human Sirtuin 5.
Format:: Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.
Extra Details:: Sirtuin-5 is a NAD-dependent protein deacetylase that activates CPS1 and contributes to the regulation of blood ammonia levels during fasting. Can deacetylate cytochrome c and a number of other proteins (in vitro). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA (direct and indirect): 1:2000-1:5000 Western Blot: 1:2000-1:5000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.