045253
Ribosomal Protein S3 (40S Ribosomal Protein S3, IMR-90 Ribosomal Protein S3, RPS3)
Size
£512.00
- SKU:
- 045253
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to human ribosomal protein S3.
- Format:
- Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.
- Extra Details:
- RpS3 is a component of the 40S ribosomal subunit and is an an essential but previously unkown subunit of NF-kappaB involved in the regulation of key genes in rapid cellular activation responses. RpS3 interacts with nm23-H1. The expression of rpS3 reduces the secretion of MMP-9 and the invasive metastatic potential in HT1080 cells. The phosphorylated ERK is reduced by the expression of rpS3. Applications: Suitable for use in ELISA, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilutions: ELISA (direct and indirect): 1:2000-1:5000 Immunocytochemistry: 1:100 Immunohistochemistry: 5ug/ml Immunoprecipitation: 1:500-1:5000 Western Blot: 1:500-1:5000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice