RELM-alpha (Resistin-like molecule-alpha, FIZZ1, PMNG1, HIMF)

Size

£416.00

SKU:: 045216
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to rat RELM-alpha.
Format:: Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.
Extra Details:: RELM-alpha is a member to the family of adipocyte-secreted proteins called adipocytokines. It is abundantly expressed in white adipose tissue but not in preadipocytes, nor 3T3-L1 adipocytes. RELM-alpha is involved in the regulation of colonic inflammation and might be a link between colonic injury, glucose tolerance, and energy intake. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA (direct and indirect): 1:5000-1:10,000 Western Blot: 1:5000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.