Polyglutamylation Modification (Polyglutamylated Tubulin, Glutamylated Tubulin, Postranslational Protein Glutamylation) (Biotin)

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£607.00

SKU:: 045160
Purity:: Purified by Protein G affinity chromatography from concentrated hybridoma tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Octapeptide EGEGE*EEG, modified by the addition of two glutamyl units onto the fifth E (indicated by an asterisk).
Clone:: GT335
Format:: Supplied as a liquid in PBS, 0.02% sodium azide. Labeled with Biotin.
Extra Details:: Polyglutamylation is a post-translational modification in which glutamate side chains of variable lengths are formed on the modified protein. It is evolutionarily conserved from and the most prominent substrate is tubulin, the microtubule (MT) building block. Polyglutamylation has been proposed to be involved in the functional adaptation of MTs, as it occurs within the carboxy-terminal tubulin tails that participate directly in the binding of many structural and motor MT-associated proteins. The recent identification of new substrates of polyglutamylation indicates that this post-translational modification could be a potential regulator of diverse cellular processes and involved in cell cycle and cell proliferation. Applications: Suitable for use in Immunocytochemistry, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications have not been tested. Recommended Dilutions: Immunocytochemistry: 1:2000 Immunohistochemistry (Paraffin): 1:1000 Western Blot: 1:4000 Optimal dilutions to be determined by researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice