Poly ADP-Ribose (PAR)

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£466.00

SKU:: 045159
Purity:: Purified by Protein A affinity chromatography from supernatant. ≥95% (SDS-PAGE)
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified poly(ADP-ribose).
Clone:: 10H
Format:: Supplied as a liquid in 50mM HEPES, pH 7.4, 100mM sodium chloride, 1% BSA, 0.02% sodium azide.
Extra Details:: Processes such as transcription, repair and replication that require efficient DNA recognition are dependent on modulation of chromatin structure. Chromatin relaxation is a critical event that occurs during DNA repair and is associated with the negatively charged polymer of adenosine 5'-diphosphate (ADP)-ribose (PAR). PAR is synthesized from nicotinamide adenine dinucleotide (NAD+) by the poly(ADP-ribose) polymerase protein family (PARPs), of which PARP-1 (and to a lesser extent PARP-2) respond to DNA-strand breaks. PARP-1 is selectively activated by DNA strand breaks to catalyze the addition of long branched chains of PAR to a variety of nuclear proteins, most notably PARP itself. The amount of PAR formed in living cells with DNA damage is commensurate with the extent of the damage. Under DNA damage conditions, PAR undergoes a rapid turnover, with a half-life in the range of minutes, as PAR is rapidly hydrolyzed and converted to free ADP-ribose by the enzyme poly(ADP-ribose)glycohydrolase (PARG). PAR regulates not only cell survival and cell-death programmes, but also an increasing number of other biological functions with which novel members of the PARP family have been associated. These include transcriptional regulation, cell division, intracellular trafficking, inflammation and energy metabolism. Applications: Suitable for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Immunocytochemistry: 5-20ug/ml Immunohistochemistry (Paraffin): 5-20ug/ml. Dilute in 5% non-fat dry milk in PBS. Western Blot: 2.5ug/ml Incubate in PBS, 0.05% Tween-20, 5% non-fat dried milk. Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

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