PIDD (p53-induced Protein with a Death Domain, Leucine-rich Repeat and Death Domain-containing Protein, Lrdd)

Size

£393.00

SKU:: 045149
Purity:: Purified by epitope affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to human PIDD (Death Domain: aa776-910).
Format:: Supplied as a liquid in PBS, 0.02% sodium azide, 10% glycerol.
Extra Details:: PIDD promotes apoptosis downstream of the tumor suppressor as component of the DNA damage/stress response pathway that connects p53/TP53 to apoptosis. It associates with NEMO/IKBKG and RIP1 and enhances sumoylation and ubiquitination of NEMO/IKBKG which is important for activation of the transcription factor NF-kappa-B. It associates with caspase-2 and RAIDD, and induces activation of caspase-2 which is an important regulator in the apoptotic pathways. Applications: Suitable for use in Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry: paraffin sections Immunoprecipitation: 1:100 Western Blot: 1:500 Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.