PEDF, Recombinant, Human (Pigment epithelium-derived factor, Cell proliferation-inducing gene 35 protein, EPC-1, Serpin F1)

Size

£429.00

SKU:: 045134
Molecular Weight:: 47
Purity:: ≥95% (SDS-PAGE). Endotoxin: ≤0.1EU/ug (LAL test; Lonza)
Storage Conditions:: 4°C/-20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder in PBS. Reconstitute with 100ul sterile ddH2O.
Extra Details:: Pigment epithelium-derived factor (PEDF) is a 47kD secreted glycoprotein that belongs to the non-inhibitory serpin family group. PEDF is widely expressed in adult and foetal tissues, including brain, spinal cord, plasma, bone, prostate, pancreas, heart and lung. PEDF acts as an angiogenesis inhibitor with neurotrophic, immunomodulation and antitumor properties. It functions as anti-angiogenic agent by counterbalancing the proangiogenic effect of VEGF. PEDF is one of the most abundant proteins released by adipocytes and induces insulin resistance in adipocytes and human skeletal muscle cells. Recently, it has been reported that PEDF is sufficient to maintain the self-renewal of pluripotent human embryonic stem cells. Sequence: Human PEDF (aa20-418) is fused at the C-terminus of to a FLAG®-tag, expressed in CHO cells. Molecular Weight: ~47kD (SDS-PAGE) Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice