LIF, Recombinant, Human (Leukemia Inhibitory Factor, Differentiation-stimulating Factor, D Factor, Melanoma-derived LPL Inhibitor)

Size

£364.00

SKU:: 045008
Molecular Weight:: 46
Purity:: ≥95% (SDS-PAGE). Endotoxin: <0.05EU/ug (LAL test; Lonza).
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, pH 7.2. Reconsitute with 100ul sterile ddH2O.
Extra Details:: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities also include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation and the stimulation of acute-phase protein synthesis in hepatocytes. LIF activates JAK & STAT signaling in human embryonic stem (ES) cells, but this pathway does not maintain pluripotency in these cells, which instead rely on FGF2-mediated ERK signaling. By contrast, mouse ES cells can be maintained by LIF-mediated JAK & STAT signaling. LIF binds to a high affinity heterodimeric receptor complex consisting of two proteins: LIF-R alpha that binds LIF with low affinity and the 130kD (gp130) subunit that by itself does not bind LIF, but is required for high affinity binding of LIF. Sequence: Human LIF (aa23-202) is fused at the N-terminus to a tag. Biological Activity: Maintains pluripotency of mouse and human embryonic stem cells. The LIF (GST-tagged) has the same activity as cleaved LIF. Specificity: Mouse and human embryonic stem cells. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice