Leptin (OB Gene Product, Obese Factor)

Size

£453.00

SKU:: 045001
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to rat leptin.
Format:: Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.
Extra Details:: Leptin is involved in the signaling pathway that regulates the size of the body fat depot. An increase in the level of leptin may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Defects in the leptin gene may be a cause of autosomal recessive obesity. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA (direct and indirect): 1:5000-1:10,000 Western Blot: 1:5000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.