IL-36 beta, Recombinant, Mouse, aa31-183 (Interleukin-36beta, Interleukin-1 Family Member 8, IL-1F8)

Size

£465.00

SKU:: 044936
Molecular Weight:: 17
Purity:: ≥95% (SDS-PAGE). Endotoxin: <0.01EU/ug (LAL test; Lonza).
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder in PBS, 0.5% trehalose. Reconstitute with 100ul sterile ddH2O.
Extra Details:: IL-36alpha (IL-1F6), IL-36beta (IL-1F8) and IL-36gamma (IL-1F9) bind to IL-36R (IL-1Rrp2) and IL-1RAcP, activating similar intracellular signals as IL-1. IL-36Ra inhibits the production of proinflammatory cytokines, including IL-12, IL-1beta, IL-6, TNF-alpha and IL-23 induced by IL-36 in BMDC and CD4 T cells. Skin and dendritic cells are targets of the IL-36 interleukins leading to a Th1 response. These cytokines may represent potential targets for immune-mediated inflammatory conditions or, alternatively, could be used as adjuvants in vaccination. Recombinant protein corresponding to Ser31-Lys183 of mouse IL-36beta, expressed in E. coli. Molecular Weight: ~17kD Biological Activity: Induces secretion of IL-6 in BMDCs at a concentration of 10-100ng/ml. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.