IDO, Recombinant, Mouse (His) (Indoleamine 2,3-dioxygenase, INDO)

Size

£554.00

SKU:: 044896
Molecular Weight:: 40
Purity:: >90% (SDS-PAGE) Endotoxin: ≤1EU/ug (LAL)
Storage Conditions:: 4°C/-20°C
Supplier:: United States Biological
Format:: Supplied as a 0.2um filtered liquid in 50mM TRIS-Cl, pH 7.4, 1mM EDTA.
Extra Details:: IDO catalyzes the first and rate-limiting step in the main pathway of human tryptophan catabolism, the kynurenine pathway. Proinflammatory mediators, such as endotoxin and IFN-gamma induce the expression of IDO in several tissues. IDO-dependent suppression of T cell responses might function as natural immunoregulatory mechanism. Physiological IDO activity has been implicated in T cell tolerance to tumors, dysfunctional selftolerance in non-obese diabetic (NOD) mice, and as a protective negative regulator in autoimmune disorders. Recombinant protein corresponding to mouse IDO (aa1-407), at C-terminus fused to His-tag. Biological Activity: Specific Activity: >100,000U/mg protein with L-tryptophan as substrate. One unit is defined as the amount of enzyme that produces 1nmol of kynurenine per hour. Heme Content: Not determined for end product. 7uM hemin was adde for protein induction. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice