Indoleamine 2,3-Dioxygenase (IDO, CD107B, INDO, Indoleamine-pyrrole 2,3-dioxygenase)

Size

£450.00

SKU:: 044892
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to human IDO.
Format:: Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.
Extra Details:: IDO catalyzes the first and rate-limiting step in the main pathway of human tryptophan catabolism, the kynurenine pathway. Proinflammatory mediators, such as endotoxin and IFN-gamma induce the expression of IDO in several tissues. IDO-dependent suppression of T-cell responses might function as natural immunoregulatory mechanism. Physiological IDO activity has been implicated in T-cell tolerance to tumors, dysfunctional selftolerance in non-obese diabetic (NOD) mice, and as a protective negative regulator in autoimmune disorders. Applications: Suitable for use in ELISA, Flow Cytometry, Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilution: ELISA (Direct and Indirect): 1:2000-1:10,000 Western Blot: 1:2000-1:5000 using ECL; Suggested blocking and dilution buffer is PBST containing 0.05% Tween-20 and 5% skim milk; Suggested incubation time is 1 hour at RT. Flow Cytometry: 1:200-1:500 Immunocytochemistry: 1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice