FTO (Fat Mass and Obesity-associated Protein, FATSO)

Size

£397.00

SKU:: 044826
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human FTO.
Clone:: FT86-4
Format:: Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.
Extra Details:: FTO (Fat mass-and obesity-associated gene) is the responsible gene for mouse 'fused toes' mutation. An association between FTO genotype and type 2 diabetes has been confirmed. The presence of the FTO rs9939609 A-allele was found to be positively correlated with other symptoms of the metabolic syndrome, including higher fasting insulin, glucose, triglycerides, and lower HDL-cholesterol. Applications: Suitable for use in ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA (direct and indirect): 1:2000-1:5000 Western Blot: 1:2000-1:5000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature Immunohistochemistry (paraffin sections): 1:2000 Immunoprecipitation: 1-5ul/sample in a final reaction volume of 500-1000ul Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice