FGF-23 (Fibroblast Growth Factor 23, Tumor-derived Hypophosphatemia-inducing Factor)

Size

£418.00

SKU:: 044808
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human FGF-23.
Clone:: FG322-3
Format:: Supplied as a liquid in PBS, pH 7.4. No preservative added.
Extra Details:: FGF-23 is a regulator of phosphate homeostasis. It upregulates EGR1 expression in the presence of KLBy. Acts directly on the parathyroid to decrease PTH secretion. Regulates the vitamin-D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization. Defects in FGF-23 are the cause of autosomal dominant hypophosphataemic rickets (ADHR) and of hyperphosphatemic familial tumoral calcinosis (HFTC). Applications: Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: ELISA (Direct or Indirect): 1:2000-1:5000 Immunohistochemistry (Paraffin): 1:100-1:500 Western Blot: 1:2000-1:5000 using ECL. Suggested blocking/dilution buffer is PBST with 0.05% Tween-20 and 5% skim milk. Suggested incubation time is 1 hour at RT. Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.