FasL, Soluble, Recombinant, Human, Flag-Tag

Size

£378.00

SKU:: 044784
Molecular Weight:: 33
Purity:: ≥95% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder in PBS. Reconstitute with 100ul sterile ddH2O.
Extra Details:: FasL is a cytokine that binds to TNFRSF6/Fas, a receptor that transduces the apoptotic signal into cells. Is involved in cytotoxic T cell mediated apoptosis and in T cell development. Sequence: The extracellular domain of human FasL (aa103-281) is fused at the N-terminus to a FLAG®-tag. Molecular Weight: ~33kD (SDS-PAGE) Endotoxin: <0.05EU/ug (LAL) Biological Activity: Induces apoptosis of human Jurkat T cells at a concentration of <1ng/ml in the presence of 0.1-1ug/ml TNF Ligands Enhancer. In the absence of TNF Ligands Enhancer, FasL is working at 50-100 fold higher concentrations. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.