DLK1 (Protein Delta Homolog 1, Fetal Antigen 1, Preadipocyte Factor 1, Pref-1, FA1)

Size

£390.00

SKU:: 044730
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corresponding to mouse DLK1 (extracellular domain).
Clone:: PF183E
Format:: Supplied as a liquid in PBS, pH 7.4. No preservative added.
Extra Details:: DLK1 (Protein delta homolog 1; Preadipocyte factor 1; Pref-1) is a regulator of adipocyte differentiation found in serum and urine. DLK1 might be a decoy ligand. Serum or plasma levels of soluble DLK1 affect negatively or positively adipogenesis and control mesenchymal cell fate. DLK1 is frequently upregulated in myelodysplastic syndrome (MDS) patients compared to non-leukemic individuals. Applications: Suitable for use in ELISA and Western Blot. Other applcations not tested. Recommended Dilutions: ELISA (Direct or indirect): 1:2000-1:10,000 Western Blot: 1:2000- 1:10,000 using ECL. Suggested blocking buffer is TBST with 0.05% Tween-20 and 5% skim milk. Suggested incubation time is 1 hour at RT. Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.