CD40, Recombinant, IgG1 Fc Human (Tumor Necrosis Factor Receptor Superfamily Member 5, TNFRSF5, CD40L Receptor)

Size

£479.00

SKU:: 044658
Molecular Weight:: 54
Purity:: ≥95% (SDS-PAGE). Endotoxin: <0.01EU/ug (LAL test; Lonza).
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder from PBS, pH 7.2. No preservative added. Reconstitute with 50ul sterile ddH2O. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
Extra Details:: CD40 belongs to the TNF-receptor superfamily and is essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development and germinal center formation. The interaction of CD40-CD40L is necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. CD40 is constitutively expressed by antigen presenting cells, including dendritic cells, B cells and macrophages. Consistent with its widespread expression on normal cells, CD40 is also expressed on a wide range of tumor cells, including non-Hodgkin's and Hodgkin's lymphomas, myeloma and some carcinomas including nasopharynx, bladder, cervix, kidney and ovary. Recombinant protein corresponding to human CD40 (aa 21-193), fused to the Fc portion of human IgG1 at the C-terminus, expressed in HEK293 cells. Molecular Weight: ~54kD (SDS-PAGE) Specificity: Binds human and mouse CD40L. Biological Activity: Blocks the binding of CD40L to CD40. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice