CD40 (Tumor Necrosis Factor Receptor Superfamily Member 5, CD40L Receptor)

Size

£416.00

SKU:: 044656
Application:: Flow Cytometry
Purity:: Purified by Protein G affinity chromatography from hybridoma tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corrresponding to mouse CD40 fusion protein.
Clone:: FGK45
Format:: Supplied as a liquid in PBS, 0.02% sodium azide.
Extra Details:: CD40 belongs to the TNF-receptor superfamily and is essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. The interaction of CD40-CD40L is necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. CD40 is constitutively expressed by antigen presenting cells, including dendritic cells, B cells and macrophages. Consistent with its widespread expression on normal cells, CD40 is also expressed on a wide range of tumor cells, including non-Hodgkin's and Hodgkin's lymphomas, myeloma and some carcinomas including nasopharynx, bladder, cervix, kidney and ovary. Applications: Suitable for use in Flow Cytometry and Functional Applications. Other applications not tested. Recommended Dilution: Functional Application: Activates B and NK cells in vivo and in vitro. For in vivo studies use 044655 (preservative free version) Optimal dilutions to be determined by researcher. Endotoxin: <0.01EU/ug (LAL test; Lonza). Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice