Calreticulin, Recombinant, Human, His-Tag (CRP55, Calregulin, Endoplasmic Reticulum Resident Protein 60, ERp60, grp60)

Size

£416.00

SKU:: 044624
Molecular Weight:: 55
Purity:: ≥90% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid from a 0.2um-filtered solution in 55mM TRIS-Cl, pH 8.2, 150mM NaCl.
Extra Details:: Calreticulin is involved in regulation of intracellular Ca2+ homoeostasis and ER Ca2+ capacity. It constitutes together with calnexin and ERp57 the 'calreticulin/calnexin cycle' that is responsible for folding and quality control of newly synthesized glycoproteins. Calreticulin has been implicated to play a role in many biological systems, including functions inside and outside the ER, indicating that the protein is a multi-process molecule. Recently, Calreticulin was shown to enhance the merger of macrophages and tumor cells, increasing phagocytosis. Sequence: Human calreticulin (aa18-417) is fused at the C-terminus to a His-tag, expressed in E. coli. Molecular Weight: ~55kD (SDS-PAGE) Endotoxin: <1EU/ug (LAL test; Lonza). Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.